ABSTRACT
F9 mouse teratocarcinoma cells have a high capacity to adhere to laminin and we identified alpha6/beta1 integrin as the principal laminin-binding protein present in these cells. F9 cells differentiated into parietal endoderm when monolayer cultures were treated with retinoic acid and dibutyryl cyclic AMP. In this process a decreased adherence to laminin was observed due to a lower expression of alpha6/beta1 integrin on the cell surface
Subject(s)
Mice , Animals , Down-Regulation , Integrins/physiology , Laminin/physiology , Tretinoin/pharmacology , Cell Adhesion , Bucladesine/pharmacology , Cell Differentiation , Flow Cytometry , Integrins/metabolism , Laminin/metabolism , Protein Binding , Receptors, Laminin/metabolism , Receptors, Laminin/physiology , Tumor Cells, Cultured/drug effectsABSTRACT
1. A new cloning procedure is described for cDNA synthesis from mRNA released by in vitro translation of polysomes in a cell-free amino acid incorporating system. The usefulness of the method lies in the feasibility of employing nanogram amounts of mRNA. 2. Complementary DNA is synthesized derectly in the translation mixture simply by adjusting the concentration of some components and removing ribosomes by boiling and centrifugation. 3. As an example, we report here the construction and characterization of cDNA clone corresponding to chick alfa (1) procollagen starting from a collagen-synthesizing polysome fraction obtained from chick embryos